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lytD [2019-08-26 08:47:07]
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lytD [2019-08-26 08:47:07]

peptidoglycan N-acetylglucosaminidase
Locus
BSU_35780
Isoelectric point
9.94
Molecular weight
95.36 kDa
Protein length
880 aa Sequence Blast
Gene length
2643 bp Sequence Blast
Function
major autolysin, cell separation
Product
peptidoglycan N-acetylglucosaminidase
Essential
no
E.C.
3.2.1.96
Synonyms
cwlG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → N-acetyl-β-D-glucosaminidases
  • Groups of genes → Secreted proteins

    • Gene

    Coordinates
    3,684,826 3,687,468

    The protein

    Catalyzed reaction/ biological activity

  • hydrolysis of the glycosidic bond between N-acetyl--d-glucosamine residues and adjacent monosaccharides PubMed
  • Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact (according to UniProt)

    • Protein family

  • glycosyl hydrolase 73 family (with LytG, according to UniProt)

    • Domains

  • SPOR 1 domain (aa 70-149) (according to UniProt)
  • SPOR 2 domain (aa 150-229) (according to UniProt)
  • SPOR 3 domain (aa 230-311) (according to UniProt)
  • SH3B domain (630-700) (according to UniProt)

    • Localization

  • extracellular (signal peptide) PubMed

    • Expression and Regulation

    Operons

    Genes
    lytD
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon
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    Biological materials

    Mutant

  • 1A788 ( lytD::tet), PubMed, available at BGSC
  • 1A792 ( lytD::tet), PubMed, available at BGSC
  • BKE35780 (lytD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT, downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC
  • BKK35780 (lytD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT, downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC

    • References

    Reviews

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    Original publications

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